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KMID : 0615720000170030151
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Jouranl of Korean Acupuncture and Moxibustion society
2000 Volume.17 No. 3 p.151 ~ p.167
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The antimutagenic effect and genetic safety of Buthus martensi Karsch aqua - acupuncture solution ( BMKAS )
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Abstract
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Objective : The aim of this study is to determine the antimutagenic effect and genetic safety of Buthus martensi Karsch aqua-acupuncture solution(BMKAS) against various chemical carcinogens.
Method : Ames(Sa(monella typhimurium) test and Rec assay(Bacillus subtilis) were used as indicators for DNA damage and anttimutagenesis. Furthermore, the levels of umu operon expression by measuring the β-galactosidase activity wete monitored with the SOS umu test using S. typhimurium 1535 containing plasmid pSK1002. And the host-mediated assay was used to investigate the mutagenicity and antimutagenicity of BMKAS inducing various chemical carcinogens after the activation with in vivo metabolic systems.
Results : From the results, BMKAS did not atfect DNA of S. typhimurium and B. subtilis strains and showed no mutagenicity at the all concentrations of tested solution. Furthermore BMKAS dose-dependently protected the mutagenecity by AF-2, 2-AA and B[a]P. These phenomena was also similar to that after metabolic activation of BMKAS in in vivo system.
Conclusion : These results suggested that BMKAS did not show the mutagenicity and protected the mutagenesis against various chemical carcinogens by four different methods used in this study.
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KEYWORD
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